| 1. | Mickael Allaire | RS | 49.64 | Athletisme Laval |
| 2. | Michael Ireland | | 50.42 | York University T.F.C. |
| 3. | Austin Adair | | 51.30 | Laurel Creek T.F.C. |
| 4. | Ryan Georgeson | 11 | 51.65 | London Legion T.F. Alliance |
| 5. | Dylan Oliveira | 11 | 51.85 | Speed Academy Athletics Club |
| 6. | Kieran Sullivan | | 52.20 | Royal City Athletics Club |
| 7. | Alan Tirop | 9 | 52.23 | Thorold Elite Track Club |
| 8. | Trace Fordham | 10 | 52.28 | Etobicoke Huskies-Striders |
| 9. | Zach Taylor | - | 52.34 | Laurel Creek T.F.C. |
| 10. | Luke Svenoy | 11 | 52.47 | University of Toronto Track Club |
| 11. | Martin Kanhai | 12 | 52.56 | Laurel Creek T.F.C. |
| 12. | Kula Nembhard | 11 | 52.71 | Brampton Racers Track & Field |
| 13. | Roger Jacobs | 11 | 52.87 | Laurel Creek T.F.C. |
| 14. | Mark Van Dalfsen | | 53.15 | Etobicoke Huskies-Striders |
| 15. | Jerome Nicholson | | 53.47 | Brampton Racers Track & Field |
| 16. | Stephen Hall | | 53.66 | Brampton Racers Track & Field |
| 17. | Noah Hill | 10 | 53.91 | Newmarket Huskies Track Club |
| 18. | Aarrush Joshi | | 53.96 | Unattached Ontario |
| 19. | Braeden Hou | - | 54.15 | Quest Sports TFC |
| 20. | Caleb Johnson | 12 | 54.29 | London Western T.F.C. |
| 21. | Frank Aguilera | | 54.42 | Lakehead Running |
| 22. | Dominic Prempeh | 11 | 54.61 | Brampton Racers Track & Field |
| 23. | Romaine McDonald | | 54.81 | Brampton Racers Track & Field |
| 24. | Evan Blake | 10 | 54.83 | Thorold Elite Track Club |
| 25. | Zayn Alibhai | 10 | 54.84 | Unattached Ontario |
| 26. | Kevin Hodgson | - | 55.08 | Durham Dragons Athletics |
| 27. | Alastair Henry | - | 55.10 | Quest Sports TFC |
| 28. | Jack Sands | | 55.32 | Royal City Athletics Club |
| 29. | William Humphrey | 10 | 55.49 | Etobicoke Huskies-Striders |
| 30. | Kyan Wright | | 55.74 | York Region Runners Club |
| 31. | Tyshawn Elgin | | 55.85 | Niagara Olympic Club |
| 32. | Chase Larsen | - | 55.91 | Thorold Elite Track Club |
| 33. | Jaden Liu | | 56.07 | Blue Devils Athletics Club |
| 34. | Kai Lemay | - | 56.28 | Blue Devils Athletics Club |
| 35. | Jase Theodore | - | 56.29 | Thorold Elite Track Club |
| 36. | Olaoluwa Adefowora | | 56.33 | Brampton Racers Track & Field |
| 37. | Muscab Mohamed | - | 56.63 | Thorold Elite Track Club |
| 38. | Owen Currie | - | 56.68 | Thorold Elite Track Club |
| 39. | Ivan Wong | | 57.02 | Blue Devils Athletics Club |
| 40. | Steven Ly | | 57.48 | Blue Devils Athletics Club |
| 41. | Henry Anigbogu | | 57.74 | Laurel Creek T.F.C. |
| 42. | Abraham Bender | | 58.02 | Unattached Ontario |
| 43. | Kieran Chan | | 58.40 | Flying Angels Academy |
| 44. | Zuhayr Abbas | | 58.60 | Saugeen Track & Field Club |
| 45. | Sai Nishant Sushil | | 58.63 | Unattached Ontario |
| 46. | Chase Owen | - | 59.78 | Athletics Niagara |
| 47. | Eoghan Heaney | | 1:00.18 | Durham Dragons Athletics |
| 48. | Pharoah Opoku-Warner | | 1:02.49 | Hamilton Olympic Club |
| 49. | Rhys Catalan | | 1:02.62 | Saugeen Track & Field Club |
| 50. | Layton Vaughan | | 1:03.26 | Royal City Athletics Club |
| 51. | Rian Hede | | 1:08.16 | York Region Runners Club |
Caleb Johnson Track & Field Result 
