| 1. | Damon Ferguson Jr | 9 | 50.55 | Ralph Durant Academy |
| 2. | Christian Barber | | 50.74 | NX Level Track Club |
| 3. | LaMont Johnson | 10 | 50.82 | Ralph Durant Academy |
| 4. | Franklin Perez | 10 | 51.68 | Lancaster Lightning TC |
| 5. | Owen Mogg | | 51.78 | Flying Angels |
| 6. | Gabriel Murray | 10 | 52.22 | Iconic Force Track Club |
| 7. | Anthony Thompson | | 52.43 | Swift Training |
| 8. | Jelonnie Blount | | 52.70 | NX Level Track Club |
| 9. | Jaylen Ball | - | 52.90 | Marlboro Boys & Girls |
| 10. | Rodney Glover Jr | 7 | 53.42 | Glenarden Track |
| 11. | Lavell Irving | - | 53.95 | NY Metropolis Track Club |
| 12. | Fabian Bolling | 10 | 54.00 | NY Metropolis Track Club |
| 13. | Jelani Taylor | - | 54.10 | Infinity Track |
| 14. | David Irabor | - | 54.15 | Iconic Force Track Club |
| 15. | Ben Bylaard | | 54.30 | Flying Angels |
| 16. | Ajani Miller | 10 | 54.33 | Southern Sprinters Elite Track & Field |
| 17. | Vance Harris III | 9 | 54.46 | Owings Mills Track |
| 18. | Isaiah Perry | | 54.53 | Swift Training |
| 19. | Daylen Scott | - | 54.69 | Bermuda Pacers Track Club |
| 20. | Marcelles Crockett | 10 | 54.76 | Capital City Blazers Track & Field Club |
| 21. | Jeremiah Parker | - | 54.94 | Sapphire - Ruff Riders Track Club |
| 22. | Ralph Etaba Ateba | 10 | 55.02 | Lightning Running |
| 23. | Dustin Bradsher | | 55.03 | Unattached |
| 24. | Stone Turnage | - | 55.08 | DC Select Track Club |
| 25. | Dmarcus Lewis | 10 | 55.11 | Bowie Boys & Girls Tracksters |
| 26. | Christopher Clements | 9 | 55.38 | USA-JA |
| 27. | Caleb Smith | - | 55.57 | Southern Sprinters Elite Track & Field |
| 28. | Kwabena Tyus | - | 55.92 | DC Select Track Club |
| 29. | Christon Raysor | 10 | 55.93 | V-Tesse Track Club of Long Island |
| 30. | Chidi Moemeka | 9 | 56.10 | Capital City Track Club |
| 31. | Terrell Mathis, Jr | 10 | 56.20 | Sapphire - Ruff Riders Track Club |
| 32. | Simeon Libby | - | 56.30 | The Greensboro Cheetahs Inc. |
| 33. | Malcolm Vaughn | - | 56.45 | DC Speed Track Club |
| 34. | Kobe Miles | 10 | 56.64 | First State Destroyers |
| 35. | Memphite Navies | - | 56.69 | Marlboro Boys & Girls |
| 36. | Deven Bailey | 9 | 57.24 | USA-JA |
| 37. | Clive Headley | 9 | 57.32 | USA-JA |
| 38. | Alonzo Coley | 8 | 57.68 | Marlboro Boys & Girls |
| 39. | Chikesandu Ogwo | 10 | 57.86 | Union Athletics |
| 40. | Albert Fenno | 15-16 | 57.87 | USA-JA |
| 41. | Kendall Hawkins-Ford | 8 | 58.66 | T4P Academy |
| 42. | London Rogers | - | 58.70 | Bowie Boys & Girls Tracksters |
| 44. | Mbaiondoum Miangar | - | 58.89 | Envy Track Club |
| 43. | William Milton | - | 58.89 | 301 Panthers |
| 45. | Tyler Knowles | - | 58.93 | Marlboro Boys & Girls |
| 46. | Tilden Travis | 9 | 59.21 | Envy Track Club |
| 47. | Shaun Johnson | - | 59.27 | DC Select Track Club |
| 48. | Derrick Gardiner | 7 | 59.34 | VELOCITY TRACK CLUB NEW YORK |
| 49. | Malachi McCaskill | 7 | 59.35 | V-Tesse Track Club of Long Island |
| 50. | Davonta Smallwood | 9 | 1:00.59 | First State Destroyers |
| 51. | Asa Muhammad | 10 | 1:00.96 | USA-JA |
| 52. | Rajon Bell | - | 1:01.39 | Glenarden Track |
| 53. | Shawford Jackson | - | 1:02.64 | Glenarden Track |
| 54. | Ethan Barnes | - | 1:04.43 | Cavalier Cavalette Track |
| 55. | Eddie Hightower Iv | - | 1:07.84 | Glenarden Track |
| 56. | Mitchell Nathan | - | 1:08.45 | Track on Point |
| 57. | Asante Moten | 9 | 1:12.38 | Capital City Blazers Track & Field Club |
Derrick Gardiner Track & Field Result 
